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Congresso Brasileiro de Microbiologia 2023
 		Resumo: 678-1

678-1

THE RNA-BIDING PROTEIN HFQ PLAYS A IMPORTANT ROLE IN THE PHYSIOLOGY AND VIRULENCE OF SALMONELLA ENTERICA TYPHIMURIUM

Autores:
Marina Floro E Silva (UNICAMP - UNIVERSIDADE ESTADUAL DE CAMPINAS ) ; Yessica Paola Jaimes Florez (UNICAMP - UNIVERSIDADE ESTADUAL DE CAMPINAS ) ; Genesy Pérez Jorge (UNICAMP - UNIVERSIDADE ESTADUAL DE CAMPINAS ) ; Marcelo Brocchi (UNICAMP - UNIVERSIDADE ESTADUAL DE CAMPINAS )

Resumo:
Salmonella enterica enterica is a well-characterized foodborne pathogen that can cause infectious diseases such as diarrhea and systemic infections. Although S. enterica is used in various study, the need to study and elucidate the mechanisms of pathogenesis is the basis for developing new treatments such as vaccines. Since the regulation of expression of genes involved in bacterial virulence is controlled by regulatory proteins, they are a useful tool for understanding these mechanisms. Hfq is one of the three major RNA-binding proteins that play an important role in regulating the global expression of the S. enterica enterica genome and have an impact on the stress response and bacterial virulence. Therefore, we did the deletion of the hfq gene performed using the lambda red recombination system as described previously using the plasmid pKD3 as a template. Generalized transduction was then performed using the P22 phage. After plasmid pCP20 was used to remove the cat gene, and the mutation was confirmed by PCR. The attenuation of the bacterial strain was assessed in vivo, using Caenorhabditis elegans and Galleria mellonella models organism. We used 20 adult worms in a plate and incubated at 25°C for 10-12 days. Groups of 10 larvae of the last star at 150-200 mg were used and were incubated at 37°C and observed for 96 hours to check the formation of pigmentation (melanization process). Our results showed that the Δhfq Δhfq mutant exhibited increased susceptibility to heat shock and osmotic stress and decreased motility. On the other hand, the deletion of the hfq gene resulted in an attenuated phenotype in C. elegans and G. mellonella models.Comparing cost and other technical requirements, C. elegans and G. mellonella may be ideal models to use as initial platforms for screening virulence attenuation of mutants.

Palavras-chave:
  Salmonella enterica, Hfq protein, virulence, model organism


Agência de fomento:
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES